We encourage you to print this article and read it at your computer, trying each step as you go. All are available for download as PDF files at While the directions and figures match the Windows version of Prism 4, all examples can be applied to Apple Macintosh systems with little adaptation. Step-by-Step Examples is one of four manuals included with Prism 4.
This article includes the following special techniques: Bar graphs created from XY graphs by converting point symbols Fitting an exponential decay curve Adding a second(right-hand) Y axis 1 Adapted from: Miller, J.R., GraphPad Prism Version 4.0 Step-by-Step Examples, GraphPad Software Inc., San Diego CA, 2003. On the same graph, we’ll graph periodic chemical measurements (activity of a tissue enzyme), shown using vertical bars. Plasma Concentration (µg/mL) 12 10 8 6 4 2 0 0 60 120 180 240 300 Pre- Injection Control Seconds In this example, we'll use point symbols and a fitted exponential decay curve to show the time course for plasma concentration of an injected drug. Version 4.0 Step-by-Step Examples Combining a Bar Graph with a Line Graph 1 Occasionally, you may wish to use both bars and lines on the same plot.
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